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Human DNA ligases can also be applicant drug targets for adjuvant cancer chemotherapy, predicated on potentiating the consequences of DNA-harming antineoplastics by transiently impeding DNA mend in tumor cells.

Armed with this particular info, it would be most informative to trace how archaeal enzymes from intently linked taxa that have incredibly comparable primary buildings arrived to differ in their nucleotide specificities, culminating by introducing amino acids modifications that elicit a achieve of operate, converting an ATP-only DNA ligase right into a twin-specificity by-product.

(24) have just lately lifted a caveat on the assertion that ADP can be an archaeal DNA ligase substrate by suggesting that contamination on the recombinant archaeal ligase protein preparations with the apparently thermostable E. coli

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DNA ligases seal 5′-PO4 and 3′-OH polynucleotide finishes by using 3 nucleotidyl transfer ways involving ligase-adenylate and DNA-adenylate intermediates. DNA ligases are critical guardians of genomic integrity, and ligase dysfunction underlies human genetic disease syndromes. Crystal constructions of DNA ligases bound to nucleotide and nucleic acid substrates have illuminated how ligase reaction chemistry is catalyzed, how ligases identify destroyed DNA ends, and how protein domain movements and Lively-site reworking are accustomed to choreograph the end-joining pathway.

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